Methodology

Large collections of mass spectra are examined with high-throughput tandem MS2 equipments. APLIPID® has libraries ready for the rapid assignment of lipid structures present in biological fluids and tissues from human and rodent sources. 

Agglomerative Hierarchical Clustering is helpful for lipidomics as shown below. The dendrogram (below) illustrates the separation of 3 categories of fatty acids, a signature of an essential fatty acid deficit associated with cystic fibrosis in children. Saturated and monounsaturated fatty acids (red colored) are increased by activated lipogenesis to compensate for the absorption deficit in dietary essential polyunsaturated omega-3 (black) and -6 (green). 

 

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